The best Side of HPLC working
The best Side of HPLC working
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Separation: The mobile phase interacts Using the stationary section from the column as well as analytes in the sample. This interaction affects how promptly Every single analyte travels from the column, leading to their separation.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
측정 가능한 농도 범위는 컬럼에 의해서도 결정됩니다. 컬럼 충진제의 종류, 입자 지름, 컬럼의 크기에 따라 분리에 최적인 시료 주입량이 크게 다릅니다.
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
-hydroxybenzoic acid elutes more slowly and gradually. Although we could resolve entirely these two solutes utilizing cellular phase that is definitely sixteen% v/v acetonitrile, we cannot solve them When the mobile period is ten% tetrahydrofuran.
The figure under shows the calibration curve and calibration equation for your list of exterior requirements. Substituting the sample’s peak location into your calibration equation provides the concentration of caffeine during the sample as 94.four mg/L.
The detector displays the eluent and generates a sign, which happens to be normally in the form of a chromatogram, which can be a graphical illustration of compound focus after a while.
, for instance, has two cell stage reservoirs which can be utilized for an isocratic elution or even a gradient elution by drawing solvents from 1 or the two reservoirs.
Bad resolution signifies analytes elute as well near together, generating them hard to differentiate. read more Here's tips on how to troubleshoot:
A pump forces a solvent through a column less than high pressures of nearly four hundred atmospheres. The column packing material or adsorbent or stationary period is often a granular product of strong particles like silica or polymers.
The HPLC column homes the stationary period, a important factor for separating analytes. Choosing the correct column is critical:
Solvent composition: The ratio of solvents website during the mobile stage might be great-tuned to improve peak resolution and separation.
Cellular stage impurities: Contaminants during the cellular period can elute through the column and display up as ghost peaks. Put together a fresh new cell period with high-purity solvents and look at filtering the mobile phase prior to use.
, that's the more frequent kind of HPLC, the stationary section is nonpolar as well as the mobile section is polar. The most common nonpolar stationary phases use an organochlorosilane in which the R group is an n